| ZHANG Yong,ZHANG Lin-sheng,GUO Chao-hua,et al.Effect of CD34+ cord blood stem cell transfected by plasmid vector pIRES2-FL-IL-3 on the mice after irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(3):228-231 |
| Effect of CD34+ cord blood stem cell transfected by plasmid vector pIRES2-FL-IL-3 on the mice after irradiation |
| Received:June 25, 2007 |
| DOI： |
| KeyWords:Interleukin-3 Flt3 ligand Stem cell Radiation Haematogenesis |
| FundProject: |
| Author Name | Affiliation | | ZHANG Yong | Department of hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China | | ZHANG Lin-sheng | Department of hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China | | GUO Chao-hua | 第三军医大学全军复合伤研究所 | | 张红宾 | Department of hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China | | 董世武 | 第三军医大学全军复合伤研究所 |
|
| Hits: 3833 |
| Download times: 2486 |
| Abstract:: |
| Objective To observe the effect of CD34+ cord blood stem cell transfected by plasmid vector pIRES2-FL-IL-3 on the mouse after irradiation and to investigate its mechanisms. Methods In the co-expressed group (12 mice), CD+34 cord blood stem cells were transfected by plasmid vector pIRES2-FL-IL-3. 5×105 cells were injected intravenously in the mouse. The hemogram changes in mice were detected 2, 4 and 6 weeks after radiation. At 6 weeks after irradiation, the expression of the CD34 in spleen was detected by immumofluorescence method. The mRNA level and the activity of IL-3 and FL were detected by RT-PCR and Western blot. Other 3 groups were CD34+ cell group (CD34 group), pIRES2-IL-3 group(IL3 group) and pIRES2-FL group(FL group),and there were 12 mice in each group. Results The survival rate of CD34 group, IL3 group and FL group at the 6th week were 25.0% (3/12), 50.0% (6/12) and 50.0% (6/12), respectively. It was 91.7%(11/12) in the co-expressed group, which was higher than those in the other groups. The expression of the CD34 of spleen in the co-expressed group was higher than those of the other groups. The mRNA level and the activity of IL-3 and FL of spleen in the co-expressed group were higher than those in the other groups too. Conclusions The CD34+ cord blood stem cells transfected by plasmid vector pIRES2-FL-IL-3 have hemogenesis promotion effect on the mice after irradiation, which was related with the aggregation, proliferation of stem cells and the high expression of the interest proteins. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
