| XU Nai-yu,ZHANG Su-ping,NIE Ji-hua,et al.Proteomic analysis of lung protein expression in rats inhaled radon[J].Chinese Journal of Radiological Medicine and Protection,2008,28(3):225-227 |
| Proteomic analysis of lung protein expression in rats inhaled radon |
| Received:May 29, 2007 |
| DOI： |
| KeyWords:Radon Lung Proteome |
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| Abstract:: |
| Objective To detect the changes in protein profile of rats' lung inhaled radon using two-dimensional electrophoresis(2-DE)and MS. Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100, 200 and 400 working level months (WLM). The total proteins of rats' lung were extracted and isolated by 2-DE. The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS. ResultsComparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots, 15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS. ConclusionsThe 2-DE image of radon groups' lung has changed, and the lung damage effect of radon is related to some proteins. |
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