| WANG Chang,WANG Guan-jun,TAN Ye-hui.Low dose radiation enhance the anti-tumor effect of high dose radiation on human glioma cell U251[J].Chinese Journal of Radiological Medicine and Protection,2008,28(3):221-224 |
| Low dose radiation enhance the anti-tumor effect of high dose radiation on human glioma cell U251 |
| Received:August 13, 2007 |
| DOI： |
| KeyWords:Low dose radiation Human glioma cell Nude mice cancer model Anti-tumor effect |
| FundProject: |
| Author Name | Affiliation | E-mail | | WANG Chang | Cancer Center of the First Hospital of Jilin University, Changchun 130021, China | | | WANG Guan-jun | Cancer Center of the First Hospital of Jilin University, Changchun 130021, China | | | TAN Ye-hui | Cancer Center of the First Hospital of Jilin University, Changchun 130021, China | wadetan@tom.com | | 姜宏宇 | Cancer Center of the First Hospital of Jilin University, Changchun 130021, China | | | 李薇 | Cancer Center of the First Hospital of Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To detect the effect on the growth of human glioma cell U251 induced by low dose irradiation and low dose irradiation combined with large dose irradiation. Methods Human glioma cell line U251 and nude mice carried with human glioma were used. The tumor cells and the mice were treated with low dose, high dose, and low dose combined high dose radiation. Cells growth curve, MTT and flow cytometry were used to detect the proliferation, cell cycle and apoptosis of the cells; and the tumor inhibition rate was used to assess the growth of tumor in vivo. Results After low dose irradiation, there was no difference between experimental group and control group in cell count, MTT and flow cytometry. Single high dose group and low dose combined high dose group both show significanly the suppressing effect on tumor cells, the apoptosis increased and there was cell cycle blocked in G\-2 period, but there was no difference between two groups. In vivo apparent anti-tumor effect in high dose radiation group and the combining group was observed, and that was more significant in the combining group; the prior low dose radiation alleviated the injury of hematological system. There was no difference between single low dose radiation group and control. Conclusions There is no significant effect on human glioma cell induced by low dose radiation, and low dose radiation could not induce adaptive response. But in vivo experience, low dose radiation could enhance the anti-tumor effect of high dose radiation and alleviated the injury of hematological system. |
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