| YU Ze-yang,FAN Wo,WU Jin-chang,Li Dong-qing,Zhu Ran,Wang Yong-qing.Effect of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on the growth and radiotherapeutic sensitivity of human lymphoma celllines[J].Chinese Journal of Radiological Medicine and Protection,2008,28(3):213-216 |
| Effect of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on the growth and radiotherapeutic sensitivity of human lymphoma celllines |
| Received:December 28, 2007 |
| DOI： |
| KeyWords:rAd-p53 p53 gene Human lymphoma cell lines Radiation therapy |
| FundProject: |
| Author Name | Affiliation | E-mail | | YU Ze-yang | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | FAN Wo | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | fanwo@public1.sz.js.cn | | WU Jin-chang | 苏州市立医院放疗科 | | | Li Dong-qing | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | Zhu Ran | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | Wang Yong-qing | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | |
|
| Hits: 3014 |
| Download times: 1882 |
| Abstract:: |
| Objective To explore the inhibitory effect and radiation sensitization of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on human lymphoma cell lines. Methods Human lymphoma cell lines Raji and Daudi were treated with rAd-p53, radiation therapy and combined treatment,respectively. The cell growth inhibition was assessed by MTT. The p53 protein expression was detected by Western blotting, and p53 mRNA was detected by RT-PCR. Results The MTT results showed that the inhibitory effect and radiosensitivity enhancement of rAd-p53 on human lymphoma cell lines were not obvious ［Raji:(27.5±4.1)%;Daudi:(28.1±1.6)%］. The results of Western blotting and RT-PCR showed that extrinsic p53 protein and p53 mRNA were expressed to some degree, but not at high-level. In addition, the results didn't demonstrate obvious radiosensitivity enhancement. Conclusions The role of inhibition and radiosensitivity enhancement of rAd-p53 was not significant on human lymphoma cell lines. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
