| FENG Wei,ZHU Shu-chai,WANG Yu-xiang.Effect of antisence VEGF on the radiosensitivity of esophageal cancer cells in vitro[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):145-148 |
| Effect of antisence VEGF on the radiosensitivity of esophageal cancer cells in vitro |
| Received:January 31, 2007 |
| DOI： |
| KeyWords:VEGF Esophageal cancer Radiosensitivity |
| FundProject:河北省自然科学基金资助项目(C2005000802),浙江省省医药卫生科学研究基金项目(C2006B011) |
| Author Name | Affiliation | E-mail | | FENG Wei | Department of Radiation Oncology,Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | ZHU Shu-chai | Department of Radiation Oncology,Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | sczhu@heinfo.net | | WANG Yu-xiang | 浙江省肿瘤医院放疗科 | | | 王玉祥 | Department of Radiation Oncology,Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | 王准 | 浙江省肿瘤医院放疗科 | | | 王鑫 | Department of Radiation Oncology,Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | |
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| Abstract:: |
| Objective To investigate the effect of antisense VEGF on the cell proliferation, VEGF protein expression and radiosensitivity of esophageal cancer cells in vitro. Methods Fragments of antisense cDNA,empty vector plasmid DNA and antisense oligodeoxynucleotide of VEGF were transfected into esophageal cancer (TE-1)cells mediated with lipofectamine, respectively. Cell proliferating rate and apoptotic rate of these groups were edetected by MTT and FCM methods, respectively. After irradiation, the expression of VEGF in transfected cells were detected by using RT-PCR and Western blotting. The radiosensitivity of transfected cells were analyzed with colony forming assay.Results After antisense cDNA plasmid and antisense oligodeoxynucleotide of VEGF were transfected successfully into TE-1 cells, expressions of VEGF protein decreased,however,the changes in cell growth rate and distribution of cell cycle, and the apoptotic rate were not observed in these transfected cells.After irradiation, the radiosensitivity of transfected TE-1 cells were increased,but there was no significant difference in cell growth rate among groups. The apoptotic rates in antisense groups increased slightly compared to TE-1 and TE-1-E groups.Conclusions Expression of VEGF mRNA and VEGF protein were significantly suppressed in TE-1 cells transfected by antisense cDNA and antisense oligodeoxynucleotide of VEGF. After irradiation, the radiosensitivity of the transfected TE-1 cells was increased. |
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