| LU Xue,FENG Jiang-bin,CHEN De-qing.Establishment of immortalized B lymphoblastoid cell lines of old residents in high background radiation area in Guangdong, China[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):139-142 |
| Establishment of immortalized B lymphoblastoid cell lines of old residents in high background radiation area in Guangdong, China |
| Received:June 09, 2007 |
| DOI： |
| KeyWords:High background radiation area Old male residents Epstein-Barr virus Immortalized cell lines B-lymphocytes |
| FundProject:国家自然科学基金资助项目(30570551);北京市自然科学基金资助项目(7053073) |
| Author Name | Affiliation | E-mail | | LU Xue | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | FENG Jiang-bin | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | CHEN De-qing | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 查永如 | 广东省职业病防治院 | | | 邹剑明 | 广东省职业病防治院 | zoujianming@gmail.com.cn | | 刘青杰 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | qjliu@nirp.cn |
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| Abstract:: |
| Objective To establish the immortalized cell lines of peripheral blood lymphocytes for old male residents in high background radiation area (HBRA) in Guangdong, China, in order to preserve the specific genomic resources of residents in HBRA for the further genetic and molecular biological study on HBRA.Methods The peripheral blood samples of 20 old male residents in HBRA were collected after informed consent. The immortalized B lymphoblastoid cell lines, 2 for each resident, were established with Epstein-Barr virus. After being frozen and recovered, the cell viability, the contamination of bacterium and mycoplasma were analyzed. The stabilization of cell lines was decided by comparing the karyotypes of the peripheral blood lymphocytes and the cell lines.Results 40 cell lines for 20 residents in HBRA were successfully established. The recovery rate of cell lines after being frozen was 100%. All the cell viablity after recovery was higher than 90%, and no contamination of bacteria and mycoplasma occurred. The karyotypes of the 20th generation cell lines were not change.Conclusion The immortalized cell lines established in this study could provide biological resources for further study on genetics and molecular biology in HBRA. |
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