| FU Chun-ling,HUO Yan-ying,HU Ying-chun.PTEN gene knock-out effect of radiosensitivity and its mechanism[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):128-131 |
| PTEN gene knock-out effect of radiosensitivity and its mechanism |
| Received:April 04, 2007 |
| DOI： |
| KeyWords:PTEN gene Radiosensitivity Reactive oxygen species AKT kinase |
| FundProject:国家自然科学基金资助项目(30471946);北京市自然科学基金资助项目(7052056) |
| Author Name | Affiliation | E-mail | | FU Chun-ling | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | | HUO Yan-ying | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | huoyy@nic.bmi.ac.cn | | HU Ying-chun | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | | 苟巧 | 重庆医科大学病理学教研室 | | | 杨柳 | 重庆医科大学病理学教研室 | | | 米粲 | 重庆医科大学病理学教研室 | | | 李刚 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | | 吴德昌 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | |
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| Abstract:: |
| Objective To analyze the effect of PTEN gene on radiosensitivity and its mechanism. Methods The reactive oxygen species levels of MEF1 and MEF1/PTEN－/－ cell were determined with flow cytometry. The AKT activity pretreated with diphenyleneiodonium chloride or hydrogen peroxide (H2O2) was detected by Western blot.Cell cloning efficiency test was used to detect the radiosensitivity. Results Deletion of PTEN increased the level of basal reactive oxygen species and decreased the radiosensitivity. Pretreatment with diphenyleneiodonium chloride or hydrogen peroxide influenced the AKT activity of control MEF1 cells but not MEF1/Pten－/－ cells. Conclusions Knock-out of PTEN gene could make AKT constitutively active and block H2O2 mediated PI3K/AKT signal transduction pathway, which should be the most reason of radioresistance. |
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