| LI Jie-qing,SONG Xian-rang,YU Jin-ming,et al.Analysis of radiosensitivity of A549 lung cancer cells with HIF-1α silencing by RNAi[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):120-123 |
| Analysis of radiosensitivity of A549 lung cancer cells with HIF-1α silencing by RNAi |
| Received:August 13, 2007 |
| DOI： |
| KeyWords:Hypoxia HIF-1α RNAi Radiosensitivity |
| FundProject:国家自然科学基金资助项目(30570547) |
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| Abstract:: |
| Objective To observe the effect of HIF-1α silencing on human lung cancer cells radiosensitivity in nude mice.Methods A549/HIF-1α(-) cell line was established by RNAi with lentivirus as vector. The expression of HIF-1α protein was analyzed by Western Blot. A549/HIF-1α(-) cells and A549 cells were injected into the male BALB/C nude mice aged 4_6 weeks.The tumors were locally irradiated with single doses (5,10,15 and 20 Gy) of X-ray irradiation when tumor sizes reached 200_350 mm3. The volumes of tumor were measured every 3 days.Time of the tumor growth delay was caculated.Results The expression of HIF-1α protein of A549/HIF-1α(-) cells was inhibited significantly under both normoxia and hypoxia condition.A549/HIF-1α(-) tumors reproduced more slowly than A549 tumors(P<0.05). X-ray radiation could inhibit tumor growth of all groups. The growth delay of A549/HIF-1α(-) tumors was more significant than of A549 tumors(P<0.05).Conclusions The expression of HIF-1α can be stably blocked by RNAi. A549/HIF-1α(-) tumors appear to be more radiosensitive than A549 tumors. |
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