| DIAO Rui-ying,SONG Liang-wen,WANG Shao-xia,et al.Exploration on remodeling of lung tissue in early radiation pulmonary injury[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):117-119,131 |
| Exploration on remodeling of lung tissue in early radiation pulmonary injury |
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| DOI： |
| KeyWords:Irradiation Fibroblast Macrophage Collagen type Ⅳ Pulmonary remodeling |
| FundProject:国家自然科学基金资助项目(30570545) |
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| Abstract:: |
| Objective To investigate the roles of collagen type Ⅳ and Matrix metalloproteinase-9 (MMP-9) on remodeling of lung tissue in early radiation pulmonary injury.Methods The proliferation of human lung fibroblast (Fb) was determined by MTT following irradiation with 60Co γ ray of 1-10 Gy; the alteration of collagen type Ⅳ and MMP-9 was measured by ELISA following Fb was irradiated with 5 and 7 Gy;Macrophage was isolated from alveolar lavage solution of rat irradiated by 25 Gy of γ ray, and condition medium of alveolar macrophage (CMAM) was prepared for stimulation of pulmonary Fb, and the cellular proliferation was determined by MTT, the synthesis of collagen type Ⅳ and MMP-9 was measured by ELISA. Immunohistochemical staining of collagen type Ⅳ and MMP-9 was performed with rat lung tissue at different times after irradiation.Results Irradiation with 1-7 Gy on pulmonary Fb could promote cellular proliferation and MMP-9 synthesis, but could not promote the collagen type Ⅳ synthesis. However, the CMAM could not only promote Fb proliferation and MMP-9 synthesis, but also promote collagen type Ⅳ synthesis and release. The deposition of collagen type Ⅳ in lung tissue could be found one week after irradiation.Conclusions Radiation can promote pulmonary Fb proliferation but can't make it produce collagen type Ⅳ. The synthesis of collagen type Ⅳ is related to interaction between pulmonary macrophage and Fb after irradiation, and it is directly involved in pulmonary remodeling after radiation pulmonary injury. |
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