| LIU Qing-jie,LU Xue,FENG Jiang-bin,et al.Comparison of BAC FISH with specific telomeres and centromere probes and chromosome painting on detection of chromosome translocation induced by irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):112-116 |
| Comparison of BAC FISH with specific telomeres and centromere probes and chromosome painting on detection of chromosome translocation induced by irradiation |
| Received:April 05, 2007 |
| DOI： |
| KeyWords:60Co γ rays Chromosome translocation BAC FISH PAINT Dose-response curve |
| FundProject:国家自然科学基金资助项目(30570551);北京市自
然科学基金资助项目(7053073);公共卫生专项(54006-0803) |
| Author Name | Affiliation | E-mail | | LIU Qing-jie | National Institute for Radiation Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | qjliu@nirp.cn | | LU Xue | National Institute for Radiation Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | FENG Jiang-bin | National Institute for Radiation Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | 王晓维 | 国家人类基因组北方
研究中心 | | | 陈德清 | National Institute for Radiation Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | |
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| Abstract:: |
| Objective To compare the efficiency of BAC FISH established in our lab and conventional chromosome painting (PAINT) on detection of radiation-induced chromosome translocation.Methods Healthy human peripheral blood samples were irradiated with 0～5.0 Gy 60Co γ-rays. Then chromosome translocations in these samples were detected with BAC FISH and PAINT using chromosomes 1, 2 and 4. The genome translocation rates were calculated with observed chromosome translocation rates, and the dose-response curve of two methods were established.Results The genome translocation rates induced by 0～5.0 Gy 60Co γ-rays detected by BAC FISH and PAINT were increased with absorbed doses. The observed translocation rates with BAC FISH were higher than that with PAINT at each dose level. The dose-response curve were Y= 0.043D2 +0.0008D +0.0048 for BAC FISH and Y=0.043D2+0.006D+0.0027 for PAINT.Conclusions The translocation rate detected by BAC FISH was higher than that by PAINT, and the parameters β in dose-response curve equation were same by two methods. |
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