| XIA Shu,ZHAO Yin,et al.Role of PI3K Akt COX-2 pathway in the radiation resistance of human cervical cancer HeLa cells[J].Chinese Journal of Radiological Medicine and Protection,2008,28(2):108-111 |
| Role of PI3K Akt COX-2 pathway in the radiation resistance of human cervical cancer HeLa cells |
| Received:June 04, 2007 |
| DOI： |
| KeyWords:PI3K Akt HeLa cell Radiosensitivity X_rays Celecoxib |
| FundProject:国家自然科学基金资助项目(30672426) |
| Author Name | Affiliation | | XIA Shu | Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China \
| | ZHAO Yin | Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China \
| | 赵茵 | Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China \
| | 郑微 | Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China \
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| Abstract:: |
| Objective To explore the role of PI3K/Akt/COX-2 pathway in the radiation resistance of human cervical cancer HeLa cells.Methods The HeLa cells were cultured in vitro. Using Celecoxib in Hela cells or associated with LY294002 for 24h, the cells were radiated by different doses of X-ray. The cell survival ratio was detected by clone formation. To calculate Dq, D0, SF2, SER, the cell survival curve was draw by one-hit multitarget model. The expression of pAkt, Akt, COX-2, Bad and pBad were detected by Western blot. The mRNA expression of COX-2 and Bad was detected by RT-PCR.Results The Dq、D0、SF2 of Celecoxib or associated with LY294002 group was lower than those in radiation group. The pathway PI3K/Akt/COX-2 was activated by irradiation. Celecoxib inhibited the activation of PI3K/Akt/COX-2 for multitarget.Conclusions The activation of PI3K/Akt/COX-2 signal transduction resulted in resistance of radiosensitization in HeLa cell. It was indicated that inhibiting PI3K/Akt/COX-2 pathway could improve the radiosensitivity of human cervical cancer HeLa cells. |
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