| FANG Liao-qiong,HE Cheng-ming,FU Xiao-lan,et al.Immunoenhancement effect of goat placenta immunoregulating factor on T cell function in immunodeficiency BALB/c mice induced by 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):46-49 |
| Immunoenhancement effect of goat placenta immunoregulating factor on T cell function in immunodeficiency BALB/c mice induced by 60Co γ-rays |
| Received:December 05, 2006 |
| DOI： |
| KeyWords:Goat placenta immunoregulating factor Radiation injury T cell function Immunoenhancement |
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| Abstract:: |
| Objective To observe the immunoenhancement effect of goat placenta immunoregulating factor (GPIF) on T cell function in mice with immunodeficiency induced by 60Co γ-rays.Methods Animal models for immunodeficiency were established with BALB/c mice whole-body irradiated by 5 Gy 60Co γ-rays. MTT method was used to evaluate the T lymphopoiesis induced by ConA. FACS were applied to analyze the expression of CD3,CD4 and CD8 in splenic lymphocytes and the rate of CD4 CD8 in thymus lymphocyte. The content of IL-2 and IFN-γin serum were measured by ELISA.Results GPIF could promote T lymphopoiesis of spleen induced by ConA and increase the levels of IL-2 and IFN-γ in serum significantly. The cell percentages of CD3+, CD4+and CD8+ lymphocytes in spleen were significantly increased, and the number of CD4+ CD8- and CD4- CD8+ groups in thymus were increased while the number of CD4+ CD8+ was distinctly decreased with GPIF applied for mice.Conclusions GPIF could facilitate T cell function and promote immunodeficiency mice recovery from radiation injury. |
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