| JIN Cheng,WU Hong,LIU Jun-ye,et al.Radiosensitization of paclitaxel-loaded nanoparticles on hypoxic MCF-7 cells[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):43-46 |
| Radiosensitization of paclitaxel-loaded nanoparticles on hypoxic MCF-7 cells |
| Received:December 08, 2006 |
| DOI： |
| KeyWords:Paclitaxel Drug delivery Nanoparticle Radiation Human breast carcinoma |
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| Abstract:: |
| Objective To prepare active and controlled paclitaxel-loaded nanoparticles and to determine the ability to radiosensitize hypoxic human breast carcinoma cells (MCF-7). Methods The poly D,L-lactide-co-glycolide (PLGA) nanoparticles containing paclitaxel were prepared with emulsification-solvent evaporation method. The drug loading efficiency, encapsulation efficiency (EE) and release profile in vitro were measured by high-performance liquid chromatography. The morphology of the paclitaxel-loaded nanoparticles was investigated by scanning electron microscopy. Cell cycle was evaluated by flow cytometry. Cell viability was measured by the ability of single cell to form colonies in vitro.Results The prepared nanoparticles were spherical shape with size between 200 and 800 nm. The drug loading efficiency and EE was 4.5% and 85.5%, respectively. The drug release pattern was biphasic with a fast release rate followed by a slow one. Co-culture of MCF-7 cells with paclitaxel-loaded nanoparticles demonstrated that released paclitaxel resulted in increase of asymmetry of cells and blocked cells in the G2 phase of the cell cycle. Paclitaxel-loaded nanoparticles effectively sensitized hypoxic MCF-7 cells to irradiation. Conclusions Paclitaxel could be effectively released from a biodegradable PLGA nanoparticle delivery system while maintaining potent radiosensitizing ability for hypoxic MCF-7 cells. |
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