| QIAO Qiao,ZHANG Shuo,CHEN Yan-zhi,et al.Role and mechanism of PKC on radiosensitization in pancreatic carcinoma cell line Panc-1[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):41-43 |
| Role and mechanism of PKC on radiosensitization in pancreatic carcinoma cell line Panc-1 |
| Received:March 16, 2007 |
| DOI： |
| KeyWords:Pancreatic neoplasm Radiosensitization Protein kinase C Apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | QIAO Qiao | Department of Radiotherapy, The First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | ZHANG Shuo | Department of Radiotherapy, The First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | CHEN Yan-zhi | Department of Radiotherapy, The First Affiliated Hospital, China Medical University, Shenyang 110001, China | | | 李光 | Department of Radiotherapy, The First Affiliated Hospital, China Medical University, Shenyang 110001, China | Liguang1963@yahoo.com.cn |
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| Abstract:: |
| Objective To explore the effect of PKC on radiosensitization in pancreatic carcinoma cell line Panc-1, and its mediating mechanism. Methods Panc-1 cells were treated with the specific activator of PKC (phorbol 12-myristate 13-acetate, PMA) and the specific inhibitor of PKC (chelerythrine, CH) to observe the SF2 changes. Cell survival was determined by clonogenic assay. The apoptosis rates of the cells were analyzed by flow cytometry with Annexin Ⅴ/PI staining. The expression of apoptosis related protein Bcl-2 and Bax after the treatment of CH and/or irradiation was determined by immunocytochemistry. Results The SF2 values of radiation group, PMA group and CH group were 0.78±0.02, 0.92±0.11 and 0.19±0.20, respectively. CH can significantly increase the sensitivity of Panc-1 to irradiation. SERs of Panc-1 cells were 1.05, 1.24 and 1.77 after the treatment of 0.5, 2 and 8 μmol/L of CH, respectively. The result of flow cytometry analysis showed that PMA decreased the apoptosis index with irradiation, while CH significantly increased the apoptosis index. Expression of Bax protein was increased significantly (P<0.05) while that of Bcl-2 was not influenced; however, the ratio of Bax/Bcl-2 was increased. Conclusions PKC regulates the radiosensitivity of Panc-1 by mediating the apoptosis of tumor cells. |
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