| CAO Zhen,XIE Cong-hua,ZHOU Fu-xiang,et al.Down-regulation of NF-κB DNA binding activity by Angelica Sinensis to ameliorate radiation-induced pulmonary injury in mouse model[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):24-27 |
| Down-regulation of NF-κB DNA binding activity by Angelica Sinensis to ameliorate radiation-induced pulmonary injury in mouse model |
| Received:May 30, 2007 |
| DOI： |
| KeyWords:Radiation-induced pulmonary injury NF-κB Angelica Sinensis |
| FundProject:国家自然科学基金资助项目(30472263);教育部博士点专项基金资助项目(20040486045) |
| Author Name | Affiliation | E-mail | | CAO Zhen | Department of Radiochemotherapy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | XIE Cong-hua | Department of Radiochemotherapy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | ZHOU Fu-xiang | Department of Radiochemotherapy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | 骆志国 | Department of Radiochemotherapy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | 韩光 | 武汉大学肿瘤防治研究中心 | | | 周云峰 | Department of Radiochemotherapy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China | yfzhouwhu@163.com |
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| Abstract:: |
| Objective To study the trend of NF-κB binding activity during the course of radiation-induced pulmonary injury (RPI), and to evaluate the intervention effect of Angelica Sinensis on it. Methods Adult female C57BL/6J mice were randomly divided into 4 groups: blank control group (N group), Angelica Sinensis control group (A group), irradiation group (NX group) and irradiation group with Angelica Sinensis intervention (AX group). All mice from the NX and AX groups underwent single fraction of 12 Gy γ-ray delivered to the whole thorax. All mice were intraperitoneally injected 25% Angelica Sinensis injection (20ml/kg) or identical volume Normal Sodiumdaily injection initiated 1 week before irradiation, lasted till 2 weeks after irradiation. Mice were sacrificed at designated time points (1, 24, 72 h, 1, 2, 4, 8, 16 and 24 weeks), and the whole lungs were removed freshly. HE and Masson staining were performed to provide histopathologic evidence and to evaluate the collagen deposit situation respectively. The immunohistochemical staining of NF-κB P65 protein was performed to identify the location as well as the relative content of P65 protein. The DNA binding activity of NF-κB was detected by TransAMTM ELISA assay.Results HE and Masson staining manifested that visible pathological alterations began at 2 weeks, typical interstitial pneumonitis were showed at 4 and 8 weeks, collagen deposition was visible from 16 weeks. The NF-κB binding activity detection and the immunohistochemical half-quantity analysis showed two-phase elevation, at 24 h and 8 weeks. Preventive application of Angelica Sinensis revealed prominently ameliorative effect for RPI, with pathological improvement, decreased immunohistochemical staining and lower NF-κB binding activity in both peaks.Conclusions During the development process of RPI, NF-κB binding activity shows two-phase elevation. Chinese medicine Angelica Sinensis can down-regulate the elevation, showing noted ameliorative effect. |
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