| DIAO Rui-ying,SONG Liang-wen,WANG Shao-xia,et al.Mechanism of IFN-γ antagonizing pulmonary fibroblast proliferation stimulated by 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):13-16 |
| Mechanism of IFN-γ antagonizing pulmonary fibroblast proliferation stimulated by 60Co γ-rays |
| Received:October 30, 2006 |
| DOI： |
| KeyWords:Human lung fibroblasts Irradiation Proliferation TGF-β1 Interferon-γ |
| FundProject:国家自然科学基金资助项目(30570545) |
|
| Hits: 3718 |
| Download times: 1860 |
| Abstract:: |
| Objective To explore the mechanism of IFN-γ antagonizing human pulmonary fibroblasts (HLF) proliferation stimulated by 60Co γ-rays.Methods The time- and dose-effect of HLF proliferation promoted by TGF-β1 and rat serum after radiation as well as effect of IFN-γ on proliferation were observed by MTT colorimetric methods. Content of TGF-β1 in rat serum and pulmonary tissues after irradiation as well as the effect of IFN-γ on rat serum after radiation promoting HLF to synthesize TGF-β1 were determined by ELISA.Results TGF-β1 played a role in promoting HLF proliferation. The sera of irradiated rats could promote HLF proliferation and TGF-β1 synthesis, but the effect was inhibited by IFN-γ significantly (P<0.05). The level of TGF-β1 in rat serum and lung tissues gradually increased from 1 to 4 weeks after irradiation.Conclusions TGF-β1 is involved in the process of γ-rays stimulating HLF proliferation, while IFN-γ can inhibit HLF from synthesizing TGF-β1 and antagonize its role of promoting HLF proliferation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
