| LIU Qing-jie,FENG Jiang-bin,ZHAO Bao-feng,et al.Detection and confirmation of two novel mitochondrial DNA deletions in human lymphoblastoid cell line irradiated by 60Co gamma rays[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):9-12 |
| Detection and confirmation of two novel mitochondrial DNA deletions in human lymphoblastoid cell line irradiated by 60Co gamma rays |
| Received:August 18, 2007 |
| DOI： |
| KeyWords:Ionizing radiation Lymphoblastoid cell line Mitochondrial DNA Deletion 7455 bp 9225 bp |
| FundProject:国家自然科学基金资助项目(30570551);北京市自然科学基金资助项目(7053073) |
| Author Name | Affiliation | E-mail | | LIU Qing-jie | National Institute for Radiological Protection, China CDC, Beijing 100088, China | qjliu@nirp.cn | | FENG Jiang-bin | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | ZHAO Bao-feng | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 陆雪 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 田梅 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 陈德清 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | |
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| Abstract:: |
| Objective To screen the radiation induced mitochondtial DNA (mtDNA) deletion and confirm it.Methods Long-range PCR with two pairs of primers, which could amplify the whole human mitochondrial genome, was used to analyze the lymphoblstoid cell line before and after exposed to 10 Gy 60Co γ-rays. The limited condition PCR was used to certify the possible mtDNA deletion shown by long-range PCR. The PCR products were purified, cloned, sequenced and the sequence results were BLASTed.Results The predicted bands of mtDNA were observed on the control cell lines, and the possible mtDNA deletions were also detected on the irradiated cell lines. The deletions were certified by the limited condition PCR. The sequence BLAST results of the cloned PCR products showed that there were two kinds of deletions, 7455 bp deletion (nt475-7929 in heavy strand) and 9225 bp deletion (nt7714-369 in heavy strand), which were both results from 8 bp direct repeats. Further bioinformatics analysis showed that the two deletions were novel deletions.Conclusions Ionizing radiation could induce the 7455 bp and 9225 bp deletions in human lymphocytes. |
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