| DING Ku-ke,SHEN Jing-jing,XU Li-li,et al.IER5 gene s mRNA expression after irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):5-8 |
| IER5 gene s mRNA expression after irradiation |
| Received:July 28, 2007 |
| DOI： |
| KeyWords:IER5 gene Irradiation AHH-1 cells HeLa cells |
| FundProject:国家高技术研究发展计划“863”项目(2004AA221160);国家自然科学基金资助项目(30770533,30400120);北京市教委科技发展项目(KM200710025007) |
| Author Name | Affiliation | E-mail | | DING Ku-ke | School of Biomedical Engineering, Capital Medical University, Beijing 100069, China | Zhoupk@nic.bmi.ac.cn | | SHEN Jing-jing | School of Biomedical Engineering, Capital Medical University, Beijing 100069, China | | | XU Li-li | School of Biomedical Engineering, Capital Medical University, Beijing 100069, China | | | 李延玲 | School of Biomedical Engineering, Capital Medical University, Beijing 100069, China | | | 周萍 | School of Biomedical Engineering, Capital Medical University, Beijing 100069, China | | | 马斌荣 | School of Biomedical Engineering, Capital Medical University, Beijing 100069, China | | | 赵增强 | 军事医学科学院辐射与放射医学研究所 | | | 隋建丽 | 军事医学科学院辐射与放射医学研究所 | | | 周平坤 | 军事医学科学院辐射与放射医学研究所 | |
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| Abstract:: |
| Objective To explore the effect of irradiation on IER5 gene expression. Methods Two kinds of cells (AHH-1 and HeLa) and the BALB/c-nu mice inoculated with tumor cells were exposed to 60Co γ-rays and analyzed by real-time PCR. The above-mentioned irradiated objects were firstly divided into groups by different doses and post-radiation time,then mRNA were extracted and reverse-transcripted to DNA before real-time PCR test.Results Under the same condition, AHH-1 was more sensitive to radiation than HeLa. The dose level corresponding to the expression peak of AHH-1 was less than that of HeLa. For AHH-1 cells,the response to 2 Gy irradiation was earlier than that to 10 Gy. But there was not remarkable difference for HeLa response between 2 and 10 Gy, and the top transcriptional levels for both cells nearly simultaneously appeared at 2 h after irradiation. In addition,the IER5 gene of human liver tumor was more sensitive than that of lung cancer and brain tumor.Conclusions IER5 might be a candidate biomarker of radiation injury, and had the potential value in radiation-therapy for liver tumor. |
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