| JIN Wen-sen,KONG Zhao-lu,SHEN Zhi-fen,et al.Study on radiation regulation of hypoxia inducible factor-1α expression and its correlation with hepatoma radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2008,28(1):1-4 |
| Study on radiation regulation of hypoxia inducible factor-1α expression and its correlation with hepatoma radiosensitivity |
| Received:July 09, 2007 |
| DOI： |
| KeyWords:Hypoxia inducible factor-1α Hepatoma cells Radiation tolerance |
| FundProject:国家高技术研究发展计划“863”项目(2002AA2Z3104);国家自然科学基金资助项目(30500143,30400089) |
| Author Name | Affiliation | E-mail | | JIN Wen-sen | The Eighth Laboratory, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | KONG Zhao-lu | The Eighth Laboratory, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | SHEN Zhi-fen | The Eighth Laboratory, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | 童顺高 | The Eighth Laboratory, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | 季华均 | The Eighth Laboratory, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | 金一尊 | The Eighth Laboratory, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | yzJin@shmu.edu.cn |
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| Abstract:: |
| Objective To study the regulation of hypoxia inducible factor-1α(HIF-1α) expression in hepatoma cells after irradiation and the expression of HIF-1α effect on the radiosensitivity of heptoma cells.Methods HepG2 cells were pretreated by Cobalt chloride (CoCl2), a chemical hypoxia agent, to induce and stabilize the expression of HIF-1α, and then exposed to different γ-irradiation doses. Clonogenic assay was used to evaluate HepG2 cell survival fraction (SF) after irradiation under normoxia and chemical hypoxia. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunoblot assay (Western blot) were utilized to detect the changes of intracellular HIF-1α on the level of transcripation and translation.Results Cell survival level was elevated by chemical hypoxia and there was a statistical difference between chemical hypoxic group and normoxic group. The ratios of SF(SFCO/SFO2)on two different conditions were increased with irradiation doses. Meanwhile, the irradiation induced up-regulation of HIF-1α in dose-dependent manner. The expression of HIF-1α was correlated with HepG2 cell survival level to some extent.Conclusions Irradiation could up-regulate the level of HIF-1α expression in HepG2 cells under chemical hypoxic condition. The cells survival level might be influenced by the changes in HIF-1α expression. |
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