| ZHAO Li,PENG Rui-yun,GAO Ya-bing.Mitochondria morphologic changes and metabolic effects of rat hippocampus after microwave irradiation[J].Chinese Journal of Radiological Medicine and Protection,2007,27(6):602-604 |
| Mitochondria morphologic changes and metabolic effects of rat hippocampus after microwave irradiation |
| Received:November 10, 2006 |
| DOI： |
| KeyWords:Microwave radiation Rat Hippocampus Mitochondria Energy metabolism |
| FundProject:军队医药卫生“十一五”专项基金(06Z065) |
| Author Name | Affiliation | E-mail | | ZHAO Li | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | PENG Rui-yun | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | pengry@nic.bmi.ac.cn | | GAO Ya-bing | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王水明 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王丽峰 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 董霁 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 徐新萍 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 马俊杰 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate the effect of microwave on mitochondria morphologic and metabolism of rat hippocampus. Methods 30 male rats were exposed to microwave with the average power density of 30 mW/cm2. Rats were sacrificed at 6 h, 1 d, 3 d and 7 d after irradiation. Electron microscope, enzymatic activity staining and spectrophotometer were used to study ultrastructure change of hippocampus mitochondria and activity of ATPase, SDH and MAO. Mitochondrial ATP, ADP and AMP contents were measured by high performance liquid chromatography (HPLC).Results At 6 h after microwave radiation, the sizes and shapes of hippocampus mitochondria were abnormal and the injury of mitochondria was aggravated at 1 and 3 d after radiation. The mitochondria presented swell, cavitation including disorder, shortness and decrease of crest. The activity of SDH and content of ATP were decreased at 6 h, most serious at 3 d(P<0.01), and recovered at 7 d after radiation. The activity of ATPase and MAO increased notably at 1 d and 3 d after radiation(P<0.01). Conclusions Microwave can damage the structure and function of mitochondria in rat hippocampus, and cause the energy metabolism of enzyme disorder. |
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