| ZHONG Bo,XU Hui,RAN Xin-ze.Changes in IFN-γ secretion in intestinal intraepithelial lymphocytes after radiation and its influence on the proliferation of IEC-6[J].Chinese Journal of Radiological Medicine and Protection,2007,27(6):537-539 |
| Changes in IFN-γ secretion in intestinal intraepithelial lymphocytes after radiation and its influence on the proliferation of IEC-6 |
| Received:November 16, 2006 |
| DOI： |
| KeyWords:Irradiation Intestinal intraepithelial lymphocytes (IEL) Rat jejunal epithelium (IEC-6) IFN-γ |
| FundProject:国家自然科学基金资助项目(30270422);国家自然科学基金重点资助项目(30230360) |
| Author Name | Affiliation | E-mail | | ZHONG Bo | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | XU Hui | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | xuhui@mail.tmmu.edu.cn | | RAN Xin-ze | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 王连友 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 粟永萍 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | |
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| Abstract:: |
| Objective To explore the changes in IFN-γ secretion in intestinal intraepithelial lymphocytes (IEL) after radiation with 60Co γ-ray of 8 Gy and its influence on the proliferation of IEC-6. MethodsELISPOT was used to detect the changes of IFN-γ secretion in IEL after radiation. The influence of IEL on the proliferation of crypt cell line from rat jejunal epithelium (IEC-6) was analyzed by MTT assay, using the cell models of IEC-6 and incorporate_ and double_cell culture system.Results IFN-γ secretion in IEL after radiation was increased. And the culture supernatant of IEL after radiation had inhibitory action on the proliferation of IEC-6. The double-cell culture system could decrease this inhibitory action, and adding anti-IFN-γ have the same effect.Conclusions IFN-γ secretion in intestinal intraepithelial lymphocytes is enhanced after radiation and it has the inhibitory action on the the proliferation of IEC-6. Cell to cell contact may be an important way for IEL to inhibit the proliferation of IEC-6. |
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