| YANG Jian,YANG Jian,WANG Chun-gang.Transfection of FHIT gene enhances the radiosensitivity of the human hepatocellular carcinoma cell line Hep G2[J].Chinese Journal of Radiological Medicine and Protection,2007,27(6):521-527 |
| Transfection of FHIT gene enhances the radiosensitivity of the human hepatocellular carcinoma cell line Hep G2 |
| Received:November 20, 2006 |
| DOI： |
| KeyWords:Ionizing radiation Fragile histidine triad Gene therapy |
| FundProject:国家自然科学基金资助项目(10575130) |
| Author Name | Affiliation | E-mail | | YANG Jian | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | YANG Jian | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | WANG Chun-gang | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | 高娴 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | 肖瑶 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | 韩玲 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | linghan8888@yahoo.com.cn |
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| Abstract:: |
| Objective To evaluate the potential of the combination of fragile histidine triad (FHIT) gene therapy and radiation treatment.Methods A plasmid (pcDNA/FHIT) containing an open reading frame of FHIT gene was estabilished,and then the plasmid was transfected into a human hepatocellular carcinoma cell line (Hep G2). Using G418 for selection, a cell line (Hep G-2/FHIT) was generated which stably expressing FHIT gene. Using cell counting kit_8 and flow cytometry,the survival fraction, cell cycle and apoptosis of the Hep G-2/FHIT cells after exposure to 60Co γ-ray were measured.Results The transfection of FHIT gene enhanced the radiosensitivity of the Hep G-2 cell. The Hep G-2 cells without FHIT showed stronger G-2 checkpoint response, which indicated that enhanced radiosensitivity was due to the FHIT gene's role in the ATR/CHK 1 pathway.Conclusions Combining γ-rays with FHIT transfection might be an effective way to treat the radioresistant cancer and FHIT might be a potential gene target for tumor treatment. |
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