| DIAO Rui-ying,SONG Liang-wen,WANG Shao-xia.Effect of collagen type Ⅳ,MMPs and TIMPs on remodeling of radiation pulmonary injury[J].Chinese Journal of Radiological Medicine and Protection,2007,27(6):517-520 |
| Effect of collagen type Ⅳ,MMPs and TIMPs on remodeling of radiation pulmonary injury |
| Received:November 30, 2006 |
| DOI： |
| KeyWords:Pulmonary injury Remodeling Collagen type Ⅳ MMPs TIMPs |
| FundProject:国家自然科学基金资助项目(30570545) |
| Author Name | Affiliation | E-mail | | DIAO Rui-ying | Institute of Radiation Medine, Academy of Military Medicine Sciences, Beijing 100850, China | | | SONG Liang-wen | Institute of Radiation Medine, Academy of Military Medicine Sciences, Beijing 100850, China | Lsong1@yahoo.com | | WANG Shao-xia | Institute of Radiation Medine, Academy of Military Medicine Sciences, Beijing 100850, China | | | 尹纪业 | Institute of Radiation Medine, Academy of Military Medicine Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the effect of collagen type Ⅳ, matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs(TIMPs) on early remodeling after radiation pulmonary injury.Methods Right lungs of rats were irradiated by 60Co γ-rays at a dose of 20 Gy to induce radiation pulmonary injury, and the lung specimens were taken at weeks 1, 2, 4 after irradiation. Quantitative analysis was performed on pulmonary collagen type Ⅳ, MMP-2, MMP-9, TIMP-2, TIMP-1 at the level of gene expression and protein synthesis using real-time PCR or immunohistochemistry.Results Gene detection using real-time PCR: gene expression of collagen type Ⅳ increased at week 1 and decreased at week 2 after irradiation; MMP-2 reached peak at week 2 in which an opposed alteration trend was displayed; MMP-9 appeared a significant trend of elevation, then decrease and elevation again which was similar to those of collagen type Ⅳ; expression of TIMP-1 was lower, and there was no marked difference among all time points; TIMP-2 displayed a trend of slight elevation, then decrease and elevation again, which was opposed to MMP-2. Immunohistochemistry-image analysis: Pulmonary collagen type Ⅳ obviously increased at week 1, and began to decrease at week 2; MMP-2 decreased at week 2 and then increased;an opposed alteration trend to that of collagen type Ⅳ was displayed; alteration trend of MMP-9 was similar to that of collagen type Ⅳ but the extent was higher; gene expression of TIMP-1 slightly increased at 2 week and an opposed trend to of MMP-9 was displayed.Conclusions Collagen type Ⅳ, MMP-2, MMP-9 and their tissue inhibitors were involved in ineffective remodeling in the early radiation pulmonary injury; MMP-2 and MMP-9 play an important role in degradation of collagen type Ⅳ; Disturbance of collagen type Ⅳ degradation might have relationship with the initiation of pulmonary fibrosis. |
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