| ZHANG Yu-song,FU Jin-xiang,ZHOU Jian-ying.Effect of Tumor Necrosis factor related apoptosis-inducing ligand(TRAIL) combined with ionizing radiation on proliferation and apoptosis of breast cancer MCF-7 cell lines[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):457-459 |
| Effect of Tumor Necrosis factor related apoptosis-inducing ligand(TRAIL) combined with ionizing radiation on proliferation and apoptosis of breast cancer MCF-7 cell lines |
| Received:October 10, 2006 |
| DOI： |
| KeyWords:Breast cancer TRAIL Ionizing radiation |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZHANG Yu-song | The 2nd Affiliated Hospital of Soochow University, Suzhou 215004, China | zhangyusongcn@yahoo.com.cn | | FU Jin-xiang | The 2nd Affiliated Hospital of Soochow University, Suzhou 215004, China | | | ZHOU Jian-ying | The 2nd Affiliated Hospital of Soochow University, Suzhou 215004, China | | | 周丽英 | The 2nd Affiliated Hospital of Soochow University, Suzhou 215004, China | | | 郭晓葵 | The 2nd Affiliated Hospital of Soochow University, Suzhou 215004, China | | | 庄志祥 | The 2nd Affiliated Hospital of Soochow University, Suzhou 215004, China | |
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| Abstract:: |
| Objective To investigate the effect of Tumor necrosis factor related apoptosis-inducing ligand (TRAIL) on breast cancer MCF-7 cell lines and the possibility of TRAIL combined with radiotherapy. Methods 1×104/ml MCF-7 cell suspension were added to each well of 96-well plates, MCF cell were treated with radiotherapy(RT), TRAIL at different concentration or RT combined with TRAIL. MTT working solution was added and calculated the inhibitory rates of MCF-7 cells. MCF-7 cell suspension was added to 6-well plates then treated with TRAIL(1 μg/ml), 8 Gy RT or TRAIL combined with 8 Gy RT. The rates of apoptosis were detected by flow cytometry after incubated 48 h. RT-PCR methods were employed to analysize the expression of apoptosis related gene in different treatment group. Results MCF-7 cell lines were resistant to TRAIL,but the inhibitory rate was upregulated when MCF-7 cell was treated with TRAIL combined with RT, which had a significant difference compared with RT or TRAIL alone. The expression of Bcl-2 and Bcl-Xl gene were down_regulated when MCF-7 cell lines was treated with 8 Gy RT combined with TRAIL. Conclusions In vitro, MCF-7 cell lines are resistant to TRAIL, but TRAIL combined with radiotherapy increased the cytotoxic effect. TRAIL has a promising prospect in clinical use. |
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