| ZHANG Ke-jun,LI De-chun,ZHU Dong-Ming.Experimental study on the effects of recombimant adenoviral-mediated mIκBα gene combined with irradiation on the treatment of hepatocarcinoma[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):454-457 |
| Experimental study on the effects of recombimant adenoviral-mediated mIκBα gene combined with irradiation on the treatment of hepatocarcinoma |
| Received:October 16, 2006 |
| DOI： |
| KeyWords:AdmIκBα gene Gene therapy Radiotherapy Hepatocarcinoma |
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| Abstract:: |
| Objective To explore the effect of recombinant adnenovirus vector mediated mutant IκBα (mIκBα) combined with radiation on the hepatocarcinoma. Methods Limited dilution method was used to test the virus titer in 293 cells. The HCC9204 cells were infected with MOI 10,20,30 and 50 for 48 h, respectively.The expression of p65 and mκB a protein was analyzed by Western blot. Transfected HCC9204 cells and controls were treated with 4 Gy γ rays. The inhibition rate of HCC9204 cells was examined by MTT. Rat modlles of HCC9204 was constructed. AdmIκBα plasmids were injected into tumor tissue and the tumors were administered with 6 Gy γ irradiation 48 hours later.Tumor growth at diferent time points was recorded during 28 days. Results The titer of AdmIκBα is 1.252×109 pfu/ml. The expression of mIκB protein was increased with titer of AdmIκBα, and p65 protein began to decrease when MOI was 10,and reached the lowest when MOI was 50, they were all dose-dependent. The proliferation of HCC9204 cell lines were suppressed, as was more significant combined with radiation,and the effect was in a viral dose-dependent manner.From days 7 to 28 after AdmIκBα gene and radiotherapy, the tumor growth was significantly slower than after irradiation or gene therapy alone. Conclusions Recombimant adenoviral-mediated mIκBα gene,combined with irradiation,can increase the cell-killing effect. It is better than that of either one alone. |
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