| RENJing-hua,LIN Ju-sheng,LIU Yao,et al.γ-H2AX assay as a measurement of DNA double-strand breaks in irradiated HepG2 cells[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):450-454 |
| γ-H2AX assay as a measurement of DNA double-strand breaks in irradiated HepG2 cells |
| Received:October 19, 2006 |
| DOI： |
| KeyWords:γ-H2AX DNA double-strand breaks Ionizing radiation |
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| Author Name | Affiliation | E-mail | | RENJing-hua | Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | LIN Ju-sheng | Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | jslin@tjh.tjmu.edu.cn | | LIU Yao | Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | 陈琼 | Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | 董旭 | Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | |
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| Abstract:: |
| Objective To explore the possibility of detecting DNA double-strand breaks (DSBs) in irradiated HepG2 cells by γ-H2AX assay and to investigate the γ-H2AX expression in different cell cycle phases. Methods HepG2 cells were blocked by TdR in G1 phase of cell cycle,and then cultured for different hours after TdR was removed. Synchronous S phase cells and G2/M phase cells were collected respectively and irradiated with 60Co γrays. Western blotting and immunofluorescence were used to analyze the expression ofγ-H2AX in cells. Results Synchronous S phase cells and G2/M phase cells were obtained at hours 34 and 40 post TdR block respectively. γ-H2AX level increased rapidly after cells were exposed to 60Co γ rays, especially in S phase of the cell cycle. Conclusions The cells in S phase are more prone to be damaged by ionizing radiation than those in G2/M.γ-H2AX assay can be regarded as an early diagnosis indicator of DSBs. |
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