| YAO Yu-shi,PAN Zhen,YU Cheng-yu.Variation of radio-sensitivity of HepG2 cells by short interferencing RNA blockage of STAT3 gene[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):444-446 |
| Variation of radio-sensitivity of HepG2 cells by short interferencing RNA blockage of STAT3 gene |
| Received:August 28, 2006 |
| DOI： |
| KeyWords:Short interferencing RNA STAT3 Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | E-mail | | YAO Yu-shi | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | PAN Zhen | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | zhenpan2003@yaho.com.cn | | YU Cheng-yu | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | 唐古生 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | 秦阳华 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | 祝宝让 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | |
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| Abstract:: |
| Objective To explore the influences on radiosensitivity of HepG2 cells after STAT3 gene inhibition by transiently transfected short interferencing RNA(siRNA). Methods Human hepatic cancer HepG2 cells were transfected with lipid coated siRNA. STAT3 gene expression and its encoding protein phosphoralation were analyzed by RT-PCR at mRNA level and by Western blotting at protein level, respectively. Radiosensitivity of HepG2 cells was evaluated using CCK-8 analysis and Hoechst 33258 DNA staining. Results STAT3 mRNA, STAT3 protein expression and its phosporalation level were obviously decreased after STAT3 specific siRNA transfection. The inhibition ratio at mRNA level was around 70%. In contrast with control group,viability of HepG2 cells was dramatically decreased when siRNA transfection was combined with 60Co γ ray(P<0.05). Conclusions Human STAT3 gene targeting siRNA inhibited the expression of STAT3 gene, and consequently decreased the activation of STAT3 protein, which increased the radiosensitivity of HepG2 cells. |
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