| LIU Fen-ju,XUE Jing,SUN Zhi-qiang,et al.Preliminary study on the mechanism of radioresistance of SHG44 cells transfected by PKB[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):441-443 |
| Preliminary study on the mechanism of radioresistance of SHG44 cells transfected by PKB |
| Received:October 16, 2006 |
| DOI： |
| KeyWords:Radioresistance PKB/AKT genes Gene transfect SHG44 cells |
| FundProject:苏州大学医学发展基金重点项目资助(EE126031);放射医学重点学科基金资助项目(ZY126001) |
| Author Name | Affiliation | | LIU Fen-ju | School of Radiation Medical and Public Health, Soochow University, Suzhou 215123, China | | XUE Jing | 苏州市立医院 | | SUN Zhi-qiang | School of Radiation Medical and Public Health, Soochow University, Suzhou 215123, China | | 杨学琴 | School of Radiation Medical and Public Health, Soochow University, Suzhou 215123, China | | 蒋亚齐 | School of Radiation Medical and Public Health, Soochow University, Suzhou 215123, China |
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| Abstract:: |
| Objective To explore the radiosensitivity of SHG44 cells increased by suppressing the protein kinase B (PKB), in order to prove whether the PKB activity is related to the radioresistance of SHG44 cells. Methods PKB gene(pCMV5.HA-m/p-PKBα(PKB),pCMV5.HA-PKBα-DD (T308D/S473D)(PKBD)) were transfected into SHG44 cells by electroporation,the cell proliferation rate was observed among the control, PKB transfected and irradiated groups by MTT assay. The laser confocal microscope was used to detect the changes of cell apoptosis and its microstructure in control,control+radiation,PKB transfected+radiation,PKBD transfected+radiation group. The proliferation of PKB transfected SHG44 cells and the relative factors of inducing apoptosis were analyzed. Results The plasmid containing extrinsic PKB was successfully transfected into SHG44 cells and expressed PKB mRNA, while there was no expression in the control group; the proliferation rate of transfected SHG44 cells was significantly different from the control group (P<0.05). 60Co γ rays could induce SHG44 cell apoptosis with the changes of cell nuclei shape. The SHG44 cells transfected by PKB in the PKB+control group were complete, with few apoptosis cells seen, while the apoptosis was more significant in PKBD+irradiation group comparing to the control-irradiation group. Conclusions SHG44 cells transfected by PKB could resist the cell apoptosis induced by radiation, suggesting that there were some relations between PKB activity and SHG44 cells radioresistance. |
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