| HAN Jing,ZHU Cai-zhong,RAN Xin-ze.Effects of irradiaiton on expression of IL-3 and IL-3Rα in Dami cells[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):430-432 |
| Effects of irradiaiton on expression of IL-3 and IL-3Rα in Dami cells |
| Received:August 04, 2006 |
| DOI： |
| KeyWords:Irradiation Megakaryocyte Cell cycle IL-3 IL-3Rα |
| FundProject:国家自然科学基金重点资助项目(30230360 |
| Author Name | Affiliation | E-mail | | HAN Jing | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | ZHU Cai-zhong | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | RAN Xin-ze | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 粟永萍 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | suyp2003@yahooo.com.cn | | 郑怀恩 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 程天民 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | |
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| Abstract:: |
| Objective To analyse the cell proliferation, cell cycle and the expression of IL-3, IL-3Rα in Dami cells after exposure to 60Co γ rays. Methods After Dami cells were exposed to 60Co γ rays, the cell cycle and proliferation of Dami cells were analyzed by means of MTT and flow cytometry. The IL-3 and the IL-3Rα expressions were detected by RT-PCR at 24 h after irradiation. Results The irradiated Dami cells showed dose-dependent proliferative inhibition and G0/G1 block. Expressions of IL-3 and IL-3Rα mRNA in Dami after irradiation at doses of 5, 10, and 15 Gy were significantly lower than those in normal Dami cells (P<0.05). Their expressions showed reverse correlation with the dose. Conclusions γ rays irradiation change the cell cycle, inhibits cell proliferation, IL-3 and IL-3Rα mRNA expression, suggesting that the abnormal expressions of IL-3 and IL-3Rα in Dami cells are involved in the injury of hemopoiesis by different doses of 60Co γ ray irradiation. |
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