| ZHANG Ping,ZHOU Zhi-guo,GAO Xian-shu,et al.Effect of recombinant adenoviruses-p53 anticancer injection (Gendicine) combined with human monoclonal antibody to EGFR (h-R3) on esophageal cancer cells with different radiosensitivities[J].Chinese Journal of Radiological Medicine and Protection,2007,27(5):427-429 |
| Effect of recombinant adenoviruses-p53 anticancer injection (Gendicine) combined with human monoclonal antibody to EGFR (h-R3) on esophageal cancer cells with different radiosensitivities |
| Received:October 18, 2006 |
| DOI： |
| KeyWords:Esophageal cancer cell lines Recombinant adenoviruses-p53 anticancer injection (Gendicine) Epidermis growth factor receptor Apoptosis |
| FundProject:国家自然科学基金资助项目(30240057);河北省自然科学基金资助项目(303508) |
| Author Name | Affiliation | E-mail | | ZHANG Ping | Department of Radiation Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | ZHOU Zhi-guo | Department of Radiation Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | GAO Xian-shu | Department of Radiation Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | xsgao777@hotmail.com | | 卢付河 | Department of Radiation Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | 宋永辉 | Department of Radiation Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | |
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| Abstract:: |
| Objective To observe the effect of recombinant adenoviruses-p53 anticancer injection (Gendicine) combined with a human monoclonal antibody to EGFR (h-R3) on esophageal cancer cells with different radiosensitivities. Methods The inhibition effect of cell growth and the influence by h-R3 and Gendicine were measured with MTT method. Changes of cell cycle and expressions of Bax and Bcl-2 after treatment with h-R3 and Gendicine were detected by FCM. Results h-R3, Gendicine and every experimental group all showed the antiproliferative effect in vitro growth profile of each esophageal cell line. Inhibition rate of the simultaneous administration was higher than h-R3 or the Gendicine group. At 12 hours after exposure to h-R3 and Gendicine, FCM analysis demonstrated that h-R3 and Gendicine treatment induced accumulation of TE13 cells in G1, with statistical significance (P<0.05). While there was a slight accumulation of TE13R120 cells in G1 phase but there was no significance (P＞0.05). At 12 hours after treatment with h-R3 and Gendicine, the pro-apoptotic Bax expression was increased and anti-apoptotic Bcl-2 expression was decreased, with no significant difference (P＞0.05). Conclusions The anti-proliferation effect of h-R3 and Gendicine was observed on different radiosensitivities esophageal cancer cells,which may be concerned with accumulation of cells in G1 phase. But the mechanism needs to be studied further. |
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