| WU Chun-ming,LI Wei-hua,LIU Rong-yao,et al.Experiment study of single low dose irradiation on subventricular zone cell[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):334-336 |
| Experiment study of single low dose irradiation on subventricular zone cell |
| Received:June 04, 2006 |
| DOI： |
| KeyWords:Rat Neural stem cells X-ray Irradiation |
| FundProject: |
| Author Name | Affiliation | | WU Chun-ming | Department of Neurosurgery, The First Affiliated Hospital, Dalian Medical University, Dalian 116011, China | | LI Wei-hua | Department of Neurosurgery, The First Affiliated Hospital, Dalian Medical University, Dalian 116011, China | | LIU Rong-yao | Department of Neurosurgery, The First Affiliated Hospital, Dalian Medical University, Dalian 116011, China | | 卢军 | Department of Neurosurgery, The First Affiliated Hospital, Dalian Medical University, Dalian 116011, China |
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| Abstract:: |
| Objective To study the effects of whole brain irradiation on subventricular zone cells (SVZ) in juvenile rat. Methods Six-week-old Wistar rats, whose brains are still growing, were irradiated with single dose of 1 Gy X-ray. Their body and brain weight were measured at days 30 or 60 after irradiation. The chronological changes of the SVZ were examined at 6 h, days 7, 14, 30 or 60 after irradiation by immunohistochemistry specifically to observe the neural stem cell using anti-nestin antibodies specific for these cells. Results The rate of brain weight gain of irradiated rats significantly decreased in comparison to controls, although that of body weight gain was similar among them. Multiple apoptotic cell appeared in the SVZ at 6 h after irradiation with simultaneous reduction in nestin-positive cell (69% of the control). The cell levels recovered within a week, with the nestin-positive cells reaching maximal (180%) on day 14, returned to baseline levels within 30 days (96%) and remained unchanged for subsequent 60 days.Conclusions Single low-dose X-ray administration reversibly affected the levels of neural stem cells in the SVZ region. The result suggest that continuous multiple administration of X-ray in clinical treatment may induce irreversible changes on neural stem cells, and cause brain growth retardation or dysfunction. |
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