| CHEN Ying-zhu,TIAN Ye,BAO Shi-yao,et al.Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):328-331 |
| Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation |
| Received:July 24, 2006 |
| DOI： |
| KeyWords:Oligodendrocyte Ionizing radiation Edaravone Tissue microarray technology |
| FundProject: |
| Author Name | Affiliation | E-mail | | CHEN Ying-zhu | Department of Neurology,The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | TIAN Ye | Department of Neurology,The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | BAO Shi-yao | Department of Neurology,The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | baoshyao@hotmail.com | | 鲍欢 | Department of Neurology,The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | 张志琳 | Department of Neurology,The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | |
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| Abstract:: |
| Objective To investigate the changes of the oligodendrocyte lineage cells in the cortex following whole brain irradiation and the effects of the neotype free radical scavenger, edaravone on radiation response of oligodendrocyte in rats.Methods 120 male Sprague Dawley rats were randomly divided into sham-irradiation group, irradiation group and edaravone group. The model of whole-brain irradiation was established with exposure of the whole brain of the rats to 4 MeV X-rays with a single-dose of 10 Gy. The rats were injected intraperitoneally with edaravone at 0.3,1.0 and 3.0 mg/kg. Tissue microarray of irradiation-induced brain injury in rats was constructed. The expression of A2B5, oligodendrocyte marker 4(O4) and 2',3'-cyclic nucleotide 3'-phosphodiesterase(CNPase) in the cortex was examined by tissue microarray technology and immunohistochemistry. The positive cells were counted.Results Compared with the sham-irradiation group, the number of A2B5-positive cells increased and the number of O4, CNPase-positive cells decreased significantly at certain time in the irradiation group(P<0.05). Compared with irradiation group, A2B5-positive cells decreased significantly after edaravone treatment, while O4-positive cells and CNPase-positive cells increased significantly (P<0.05, or P<0.01).Conclusions The number of oligodendrocyte precursor cells in the cortex of rats increased reactively following whole brain irradiation and changed with time. Edaravone played a protective role in oligodendrocyte ischemic reaction in a dose-dependent manner. |
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