| LI Hai-jun,LE Chen,TIAN Xiao-li,et al.Observation of adhesion molecules expression and its adhesion ability in human peripheral blood cells exposed to γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):325-328 |
| Observation of adhesion molecules expression and its adhesion ability in human peripheral blood cells exposed to γ-rays |
| Received:July 04, 2006 |
| DOI： |
| KeyWords:Peripheral blood leucocyte cell γ-ray Adhesion molecules |
| FundProject: |
| Author Name | Affiliation | E-mail | | LI Hai-jun | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China | | | LE Chen | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China | | | TIAN Xiao-li | 东莞中山大学附属东华医院产科 | | | 张军 | 第二军医大学长海医院检验科 | | | 姜昊 | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China | | | 闵锐 | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China | minrui021@hotmail.com |
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| Abstract:: |
| Objective To study the adhesion molecules expression in leucocytes and its adhesion ability in human peripheral blood mononuclears(PBMC) exposed to γ-ray within 24 hours.Methods Heparinized human peripheral blood samples were exposed to γ-rays with 0, 2, 4 and 6 Gy. Then flow cytometry was used to examine the expression of adhesive molecules such as CD11a, CD11b, CD18, CD29, CD49d and CD54 in cells. Cell adhesion analysis was performed to determine the adhesion abilities to different substance of PBMC at 6, 12 and 24 h after irradiation.Results At 6 h after exposure, CD29 expression was increased in PBMC in 2 and 4 Gy dose groups, but decreased at other time and doses checking points. A dose-dependent increase in adhesion ability of PBMC was observed in IgG-coated plates at 6, 12 and 24 h after irradiation. Conclusion A good relationship was observed between CAMs expression, cell adhesive ability and irradiation dose level. |
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