| WANG Dong,QING Yi,ZHONG Zhao-yang,et al.Experimental study of APE1 RNA interference enhancing the sensitivity of neutron radiation in osteosarcoma[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):322-324 |
| Experimental study of APE1 RNA interference enhancing the sensitivity of neutron radiation in osteosarcoma |
| Received:July 31, 2006 |
| DOI： |
| KeyWords:Bone tumor APE1 252Cf Neutron Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | E-mail | | WANG Dong | Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China | dongwang64@hotmail.com | | QING Yi | Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China | | | ZHONG Zhao-yang | Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China | | | 李增鹏 | 第三军医大学大坪医院野战外科研究所肿瘤中心, 病理科 | | | 张沁宏 | 第三军医大学大坪医院野战外科研究所肿瘤中心, 病理科 | | | 杨宇馨 | Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China | |
|
| Hits: 3696 |
| Download times: 2613 |
| Abstract:: |
| Objective To knock down APE1 gene expression in HOS cells, and explore its antitumor effects in combination with 252Cf neutron radiotherapy.Methods pSilence APE1 siRNA plasmid was transfected into HOS cells by SuperFect Transfection liposome. The transfected HOS cells were irradiated by 252Cf neutron, then MTT assay, clone formation assay and alkaline comet assay were used to detect the radiobiological reaction, and cell apoptosis was detected with flow cytometry.Results The D37 value was 3.02 vs. 2.42 in the control and transfected HOS cells respectively after irradiation with 252Cf neutron, the DMF value is 1.43. The tail moments and cell apoptosis rate at 200, 500 and 1000 cGy showed significant difference between the two groups (P<0.05).Conclusions pSilence APE1 may significantly sensitize the neutron radiotherapy of the HOS cells, and enhance DNA damage and cell apoptosis. Therefore, the gene therapy with pSilence APE1 combined 252Cf neutron radiotherapy may be a promising approach to therapy of human osteosarcoma in the future. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
