| HAN Guang,ZHOU Yun-feng,WANG Zhao-hua,et al.Expression of ICAM-1 in mice with radiation induced lung injury[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):318-321 |
| Expression of ICAM-1 in mice with radiation induced lung injury |
| Received:November 06, 2006 |
| DOI： |
| KeyWords:Radiation-induced lung injury ICAM-1 Hydroxyproline Leukocyte common antigen |
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| Abstract:: |
| Objective To observe the expression of intercellular adhesion molecule-1(ICAM-1) in mice with radiation induced lung injury and to study the function of ICAM-1.Methods The thoraces of C57BL/6 mice were exposed to either sham irradiation or single fraction of 12 Gy. Two groups were defined as received sham-irradiation (C group) and underwent irradiation (X group). Mice were sacrificed at hours 1, 24, 72 and weeks 1, 2, 4, 8, 16, 24 after irradiation. The lung tissues were removed and processed for definitive analysis, including HE and Masson staining, the hydroxyproline content, the immunohistochemistry and the real-time quantitative RT-PCR.Results Compared with C group, there was a significant histological and pathologic change in X group. And there was a significantly elevated level of positive cell counts of ICAM-1 and inflammatory cells in X group (P<0.01). Similarly, there was a significantly elevated level of hydroxyproline in X group(P<0.05). Moreover, the results of real-time quantitative RT-PCR showed that the relative mRNA expression of cytokine ICAM-1 in X group was significantly higher than that of C group(P<0.01). ConclusionsAs an important cytokine in radiation-induced lung injury, ICAM-1 can not only mediate the inflammation cells adherence and infiltration, but also be involved in radiation induced lung fibrosis. |
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