| LIAO An-yan,WANG Jun-jie,ZHAO Yong,et al.Study on apoptosis of prostate cancer cell induced by 125I seed irradiation[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):311-313 |
| Study on apoptosis of prostate cancer cell induced by 125I seed irradiation |
| Received:March 08, 2007 |
| DOI： |
| KeyWords:125I seeds Prostate cancer cell Apoptosis Caspase-3 Bcl-2 |
| FundProject: |
| Author Name | Affiliation | E-mail | | LIAO An-yan | Cancer Center, Peking University Third Hospital, Beijing 100083, China | | | WANG Jun-jie | Cancer Center, Peking University Third Hospital, Beijing 100083, China | junjiew-@263.net | | ZHAO Yong | 中国科学院动物研究所生物膜国家重点实验室 | | | 王济东 | Cancer Center, Peking University Third Hospital, Beijing 100083, China | | | 庄洪卿 | Cancer Center, Peking University Third Hospital, Beijing 100083, China | |
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| Abstract:: |
| Objective To explore the mechanism of apoptosis induced by 125I seed irradiation on PC3 cells.Methods Human prostate cancer cell line PC3 was treated by iradiation of 125I (2.77 cGy/h) with various dose. Agarose gel electrophoresis of DNA and flows cytometry were used to detect the apoptosis of PC3 cells and indirect immunofluorescence assay was used to detect the expression of Bcl-2.The activity of Caspase-3 was measured by Caspase Colorlmetric Assay Kits.Results Apoptosis of PC3 cells could be efficiently induced by 125I seed irradiation.The apoptotic peaks were found by flow cytometry and DNA ladder appeared on 1.8％ agarose ge1.The activity of Caspase-3 on PC3 cells treated by 125I seed irradiation was not changed significantly.Bcl-2 gene expression was down-regulated with the sample concentration increased. Conclusion 125I irradiation can induce the apoptosis of PC3 cells and the mechanism of apoptosis is related with down regulation of Bcl-2 gene expression and is not related with Caspase-3 activity. |
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