| ZHU Yan,HUANG Li-fang,LUO Xiao-hua,et al.Effect of irradiation on APN/CD13 enzymatic activity on AGM stromal cells[J].Chinese Journal of Radiological Medicine and Protection,2007,27(4):307-310 |
| Effect of irradiation on APN/CD13 enzymatic activity on AGM stromal cells |
| Received:November 06, 2006 |
| DOI： |
| KeyWords:APN/CD13 AGM region Stromal cells Irradiation-induced injury Enzymatic activity |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZHU Yan | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | HUANG Li-fang | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | LUO Xiao-hua | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | 孙汉英 | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | Shanhum@163.com | | 冉丹 | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | 张可杰 | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | 郑邈 | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | 周琨 | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | | | 刘文励 | Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China | |
|
| Hits: 3590 |
| Download times: 2227 |
| Abstract:: |
| Objective To investigate the expression of APN/CD13 in mice embryo AGM stromal cells and the change of its enzymatic activity before and after irradiation.Methods The expression of APN/CD13 in AGM stromal cells was assayed by RT-PCR and immunohistochemistry. The stromal cells in AGM region were irradiated with 8.0 Gy of 60Co γ-rays, and APN/CD13 enzymatic activity was measured by spectrophotometer at different time points.Results The APN/CD13 expression level was enhanced significantly in AGM stromal cells. The enzymatic activity of APN/CD13 decreased temporally post-irradiation injury, then increased to the highest level 4 hours post-irradiation, and it returned to the level of before irradiation 24 to 48 hours post-irradiation.Conclusions The expression of APN/CD13 was remarkable in AGM stromal cells. The enzyme activity of APN/CD13 was temporally enhanced after irradiation, which might be one of the compensatory mechanisms to promote the hematopoietic recovery after irradiation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
