| ZHUANG Liang,YU Shi-ying,HUANG Xiao-yuan,et al.Response of cervical carcinoma and normal interstitial cell lines after X-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2007,27(3):241-243 |
| Response of cervical carcinoma and normal interstitial cell lines after X-ray irradiation |
| Received:July 04, 2006 |
| DOI： |
| KeyWords:Cervical carcinoma Irradiation Apoptosis G2/M phase block |
| FundProject: |
| Author Name | Affiliation | | ZHUANG Liang | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | YU Shi-ying | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | HUANG Xiao-yuan | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 李小兰 | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 熊华 | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 郑祖安 | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 冷彦 | Cancer Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract:: |
| Objective To study the response of cervical carcinoma,normal vascular endothelial and fibroblast cell lines simulated as cervical carcinoma tissue after different doses of X-ray irradiation. Methods Human cervical adenocarcinoma cell line HeLa, human cervical squamous carcinoma cell line SiHa, C33A and Caski, human umbilical vein endothelial cell line ECV304 and mouse fibroblast cell line NIH/3T3 were irradiated with 6 MV X-ray at the doses of 6 Gy and 10 Gy. Apoptotic rate and cell cycle of these cells were analyzed by flow cytometry 24 h and 48 h after irradiation. Results Among the cell lines, the apoptotic rate of C33A was the highest, and the SiHa cell line was most resistant to X-ray, while the apoptotic rates of the rest were between C33A and SiHa cell lines after 6 Gy and 10 Gy irradiation. The apoptotic rate of ECV304 after irradiation with 10 Gy (13.04%±1.08%) was higher than that of 6 Gy (6.51%±0.61%,P=0.001). Six cell lines were all blocked at G2/M phase after irradiation, the percentages of G2/M phase increased with the augment of irradiation doses and arrived at the maximum at 24-48 h after irradiation. Conclusion High dose irradiation could enhance the apoptotic rate of vascular endothelial cell and increase the cell percentage of G2/M phase in all cell lines, which provided the references for radiotherapy of cervical carcinoma at high dose of radiation. |
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