| ZHOU Li,WU Cong-mei,HUANG Tian-hua.Enhancement of pEgr-p16 expression induced by irradiation and its anti-tumor effect on B16 melanoma cells in vitro[J].Chinese Journal of Radiological Medicine and Protection,2007,27(3):235-237 |
| Enhancement of pEgr-p16 expression induced by irradiation and its anti-tumor effect on B16 melanoma cells in vitro |
| Received:October 09, 2006 |
| DOI： |
| KeyWords:pEgr-p16 gene transfection Irradiation Melanoma B16 cells |
| FundProject:广东省科技计划资助项目(2003C30304),汕头市重点科技计划资助项目(汕府科[2006]82号) |
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| Abstract:: |
| Objective To study the antitumour effect of irradiation combination with recombined pEgr_p16 plasmid on melanoma B16 cells.Methods pEgr-p16 plasmids were transfected into B16 cell line. Quantitative RT-PCR, flow cytometry and MTT methods were used to detect the expression of p16, cell apoptosis and inhibition effect,respectively.Results The p16 expressions in different doses X-ray irradiation group were about 3.78-6.67 times higher than that in sham_irradiation group (P<0.01). The expressions of p16 gradually increased with time after exposure to 2 Gy X-ray irradiation and reached to maximum at 72 h. Apoptosis rate in transfected p16 combined with irradiation group was higher than that in either plasmid or irradiation group alone (P<0.05-0.01). The number of pEgr-p16-transfected B16 cells exposed to 2Gy X-ray irradiation was significantly less than those in other experimental groups (P<0.05-0.001).Conclusions pEgr-p16 gene transfection combined with irradiation could suppress melanoma B16 cell growth.And the inhibition was more effective than those in gene- or irradiation-therapy alone. |
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