| LIU Xiao-dong,LIU Yang,ZHAO Yin-long,et al.Ionizing radiation_induced adaptive response in human fibroblasts under 3_dimentional culture condition[J].Chinese Journal of Radiological Medicine and Protection,2007,27(3):215-218 |
| Ionizing radiation_induced adaptive response in human fibroblasts under 3_dimentional culture condition |
| Received:May 22, 2006 |
| DOI： |
| KeyWords:Ionizing radiation 3_dimentional culture Adaptive response |
| FundProject:国家自然科学基金资助项目(30500142);教育部留学回国人员科研启动基金(2005年);吉林省科技厅基金(2005136);吉林大学创新基金(2004年) |
| Author Name | Affiliation | E-mail | | LIU Xiao-dong | MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China | | | LIU Yang | MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China | | | ZHAO Yin-long | MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China | | | 马淑梅 | MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China | shmm2001@yahoo.com.cn |
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| Abstract:: |
| Objective To observe the adaptive response induced by ionizing radiation in human fibroblasts under 3_dimentional culture condition.Methods Human fibroblasts were cultured under 3_D condition.Western blot was used to detect the expressions of p53 and p21.Immunofluroescence and confocal microscopy were used to detect the morphological changes.Results The structures of the cells under 2_D and 3_D conditions were fundamentally different,the cells under 3_D condition stretched out and formed microspikes,the proportions of normal, abnormal and in between were 87.05%,6.1% and 6.85%,respectively.After the treatment of 2 Gy(D2) most of the fibroblasts shrinked and the microspikes disappeared,while the pre_tretment of 0.1 Gy(D1) pulsing the challenging dose of 2 Gy(i.e.,D1+D2) caused the decrease of abnormal cells and the increase of the normal cells and in between(35.5%:34.7%:30.4%).Conclusions Under the 3_D conditions pretreatment with low dose radiation could attenuate the morphological changes induced by high dose.i.e. induced the adaptive response. |
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