| ZHU Xiao-dong,QU Song,LI Dan-rong.Relationship between the expression of Ku gene and radiosensitivity in nasopharyngeal carcinoma cell strains[J].Chinese Journal of Radiological Medicine and Protection,2007,27(2):128-131 |
| Relationship between the expression of Ku gene and radiosensitivity in nasopharyngeal carcinoma cell strains |
| Received:March 17, 2006 |
| DOI： |
| KeyWords:Nasophryngeal carcinoma cell lines Ku gene Radiosensitivity |
| FundProject:广西自然科学基金资助项目(0575090) |
| Author Name | Affiliation | | ZHU Xiao-dong | Department of Radiation Oncology, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | | QU Song | Department of Radiation Oncology, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | | LI Dan-rong | Department of Radiation Oncology, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | | 曹骥 | Department of Radiation Oncology, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | | 张玮 | Department of Radiation Oncology, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China |
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| Abstract:: |
| Objective To elucidate the relationship between expression of Ku70/Ku80 gene (a modulating subunit of DNA-PK,which is an important component in NHEJ pathway) and radiosensitivity of nasopharyngeal carcinoma cell strains. Methods Radiosensitivity parameters of nasophryngeal carcinoma cell strains were obtained by the colony forming assay and dose-survival curve was developed according to linear quadratic model. The two cell strains were exposed to 4 Gy of 60Coγ rays, the growth rate of cell group was obtained by MTT assay, and the different radiosensitivity of two cell strains was evaluated. The dynamical changes of the expression of Ku70 and Ku80 in mRNA level in CNE-1 and CNE-2 were detected by RT-FQ-PCR at different doses. Results The survival rates of CNE-1 were higher than those of CNE-2 at each dose point. The mean inactivation dose was higher in CNE-1(2.78) than that in CNE-2(1.61),and the values of SF2 was 0.627 and 0.341, respectively. After 4 Gy irradiation, the survival rate in CNE-1 was also higher than that in the CNE-2. The ratio of relative expression of Ku70 and Ku80 in the two cell strains was 1.76±0.54(P=0.07)and 13.82±3.78(P=0.004),respectively. The expression of Ku80 in CNE-2 was correlated with the irradiation dose. Conclusions CNE-2 cells are more radiosensitive than CNE-1. The expression of Ku80 gene is correlated with the radiosensitivity of nasophryngeal carcinoma cells. |
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