| JIN Wei,CUI Yu-fang,AN Xiao-xia,et al.Effects of γ-rays on Th1 and Th2 immune function of mice[J].Chinese Journal of Radiological Medicine and Protection,2007,27(2):124-127 |
| Effects of γ-rays on Th1 and Th2 immune function of mice |
| Received:April 25, 2006 |
| DOI： |
| KeyWords:Th1 Th2 γ-rays mice Flow cytometry |
| FundProject:总后“十五”基金资助项目(01MA075 ,02M010);总后“十一五”基金资助项目(06Z064) |
| Author Name | Affiliation | E-mail | | JIN Wei | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | CUI Yu-fang | Beijing Institute of Radiation Medicine, Beijing 100850, China | yufangc@vip.sina.com | | AN Xiao-xia | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 徐菡 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 董波 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 柳晓兰 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 罗庆良 | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To observe the effects of 6 Gy whole body γ-irradiation on immune function of Th1 and Th2 in mouse, and to investigate the cellular and molecular mechanism of immune system injury induced by irradiation. Methods Surface marker and intracellular cytokines of lymphocytes were stained with fluorescence-labeled monoclonal antibodies, then the changes of lymphocyte subpopulations, especially the Th1 and Th2 in mouse peripheral blood and spleen were analyzed by flow cytometry. Results (1) 1 d after 6 Gy γ-irradiation, lymphocytic subsets of CD19+ and CD8+ in spleen decreased apparently and the percentages of them were only 30% and 41% of control groups respectively(P<0.01). The percentages reached the minimum at 7 d for CD19+ and 14d for CD8+ respectively,however, up to 21d post-irradiation they still did not return to control level. (2) Th1 subpopulations in mouse peripheral blood and spleen were significantly reduced at 1 d after irradiation and were only 2.6% and 7.6% of control groups(P<0.01), while Th2 cells were not decreased and instead increased to some extent in peripheral blood at 7 d post-irradiation. (3) It was found the ratio of CD4+/CD8+were significantly increased at 1 d post-irradiation in mouse spleen because of swift reduction of CD8+cells. Interestingly, either in peripheral blood or in spleen in irradiated mice, the ratio of Th1/Th2 were evidently raised because of the decrement of Th1 cells, exhibited obviously a phenomenon of predominant immune response of Th2 cells. Conclusions It is suggested that the depression of mouse immune function induced by 6 Gy γ-irradiation might be caused by changes of CD4+/CD8+ ratio, especially the imbalance of Th1/Th2 function subpopulations. It is shown that the imbalance of Th1/Th2 function subpopulations plays an important role in radiation-induced immune injury, thus providing a better insight into the molecular mechanism and new strategies for prevent and treatment measures of immune injury by irradiation. |
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