| TAO Si,SUN Han-ying,LIU Wen-li.Effect of cotransplantation of hematopoietic stem cells and embryonic AGM stromal cells on hematopoietic reconstitution in mice after bone marrow transplantation\[J].Chinese Journal of Radiological Medicine and Protection,2007,27(2):117-120 |
| Effect of cotransplantation of hematopoietic stem cells and embryonic AGM stromal cells on hematopoietic reconstitution in mice after bone marrow transplantation\ |
| Received:February 28, 2006 |
| DOI： |
| KeyWords:Aorta-gonad-mesonephros (AGM) region Syngeneic bone marrow transplantation Hematopoietic reconstitution Hematopoietic microenvironment |
| FundProject:国家自然科学基金资助项目(C03030202) |
| Author Name | Affiliation | E-mail | | TAO Si | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | SUN Han-ying | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | hanyingsun@hotmail.com | | LIU Wen-li | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | |
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| Abstract:: |
| Objective To explore the effects of cotransplantation of hematopoietic stem cells and stromal cells derived from aorta-gonad-mesonephros (AGM) region on hematopoietic reconstitution in mice after bone marrow transplantation (BMT). Methods The typical mice model of syngeneic BMT was established and the mice were randomly divided into 4 groups: the control group, the BMT group, the group of cotransplantation of HSC with AGM stromal cells (the cotransplantation group) and the ligustrazine group (the LT group). On days 3, 7, 10, 14, 21 and 28 after BMT, the peripheral blood cells and bone marrow mononuclear cells (BMMNC) were counted, and histology changes of bone marrow were detected. Results The levels of peripheral WBC, RBC, platelet, and BMMNC in the contransplantation group were significantly higher than those in the single BMT group and the LT group (P<0.05). Conclusions Cotransplantation with AGM stromal cells could significantly promote hematopoietic reconstruction in mice after BMT. |
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