| ZHAO Bao-feng,TIAN Mei,RUAN Jian-lei.Observation on bio-effect of EJ cell line with smac gene transferred and 12C6+ ions-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2007,27(1):67-69 |
| Observation on bio-effect of EJ cell line with smac gene transferred and 12C6+ ions-ray irradiation |
| Received:July 31, 2006 |
| DOI： |
| KeyWords:Smac gene Carbon ions Radiotherapy EJ cell Apoptosis A:空白对照组 B:转染空载体组 C:转染smac基因组 图1 免疫组化检测的EJ细胞内smac基因表达情况 ×200 |
| FundProject:中国博士后基金资助项目(2005038364);兰州重离子加速器国家实验室基金资助项目 |
| Author Name | Affiliation | E-mail | | ZHAO Bao-feng | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | TIAN Mei | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | RUAN Jian-lei | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 苏旭 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | suxu@nirp.cn | | 李文建 | 中国科学院近代物理研究所 | |
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| Abstract:: |
| Objective To observe whether smac gene can increase the bio-effect of EJ cells induced by 12C6+ ions irradiation. Methods The cells were divided into three groups: blank control group, vector transferred group and smac gene transferred group. Immuno-histochemistry and flow cytometry were used to identify the expression of smac gene. Following 12C6+ ions treatment at 0, 2, 4 Gy, three groups' survival ratio was detected by colony-formation assay. 24 h after 12C6+ ions treatment, flow cytometry was used to detect apoptosis. Results Immuno-histochemistry results indicated that the expression of smac gene in smac gene transferred group was notably enhanced compared with vector pcDNA3.1 transferred group. Fluorescence-activated cell sorting (FACS) results also indicated that the expression of smac gene in smac gene transferred group was significantly enhanced compared with that of vector pcDNA3.1 transferred group (3080 vs 2256,P<0.05), but there is no significant difference between vector pcDNA3.1 transferred group and blank control group. The results of colony-formation assay suggested that the smac gene transferred group showed significantly lower survival fraction than vector pcDNA3.1 transferred group (P<0.05). 24 h after 12C6+ ions treatment, FACS results indicated that smac gene transferred group showed apparent cell apoptosis ratio, which was higher than that of vector pcDNA3.1 transferred group (P<0.05). Conclusions Smac gene could show high expression in smac gene transferred group cells and increase the apoptosis ratio of EJ cells. Smac gene could further enhance the apoptosis and notably decrease the survival fraction of EJ cells when combined with 12C6+ ions irradiation. |
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