| GUO Jun-bing,FENG Chong-jin,JIANG Hong-wei.Expression of cyclooxygenase-2 in radiation-induced oral mucositis in rats[J].Chinese Journal of Radiological Medicine and Protection,2007,27(1):63-66 |
| Expression of cyclooxygenase-2 in radiation-induced oral mucositis in rats |
| Received:March 28, 2006 |
| DOI： |
| KeyWords:Cyclooxygenase-2 Radiation Oral mucositis |
| FundProject:广东省科技计划基金资助项目(2004B34001003;2004B30901012) |
| Author Name | Affiliation | E-mail | | GUO Jun-bing | Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China | | | FENG Chong-jin | Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China | drfcj@yahoo.com.cn | | JIANG Hong-wei | 510080 广州, 中山大学附属口腔医院 | | | 李春阳 | 510080 广州, 中山大学附属口腔医院 | | | 程斌 | 510080 广州, 中山大学附属口腔医院 | | | 陈勇 | 510080 广州, 中山大学附属肿瘤医院放疗科 | | | 王汉渝 | 510080 广州, 中山大学附属肿瘤医院放疗科 | | | 李蕙君 | Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China | |
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| Abstract:: |
| Objective To detect the mRNA and protein levels of COX-2 in the tissues with radiation-induced oral mucosa(ROM) in rats and explore the mechanism of COX-2 in ROM. Methods The Sprague-Dawley (SD) rat model of ROM was established. Both sides of buccal mucosa were excised. The cDNA of COX-2 was synthesized using the semi-quantitative RT-PCR. The Streptavidin-Biotin-Complex Method (SABC Method) was employed to determine the localization, intensity and distribution of COX-2 protein expression. Results The SD rat model of ROM was established. The results of RT-PCR showed that mRNA of COX-2 could be expressed in the left buccal mucosa but rarely in the right buccal mucosa. Immunohistochemical analysis of COX-2 demonstrates that COX-2 protein can be expressed in the left side mucosa. Conclusions The mRNA and protein of COX-2 can be expressed in the mucosa of ROM. The expressions of COX-2 have correlation with the severity of ROM. |
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