| XU Rui-ming,WU Ning,LIU Yang.Effect of whole-body X-irradiation on the expression of protein kinase C θ in immune cells in mice[J].Chinese Journal of Radiological Medicine and Protection,2007,27(1):60-62 |
| Effect of whole-body X-irradiation on the expression of protein kinase C θ in immune cells in mice |
| Received:November 28, 2005 |
| DOI： |
| KeyWords:Ionizing radiation Membrane-translocation PKC θ T cell |
| FundProject:国家自然科学基金资助项目(30100033) |
| Author Name | Affiliation | E-mail | | XU Rui-ming | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | | WU Ning | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | | LIU Yang | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | | 金顺子 | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | shunzij@yahoo.com.cn |
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| Abstract:: |
| Objective To observe the effect of ionizing radiation on protein kinase C θ (PKC θ) expression in immune cells from thymus and spleen in mice. Methods Avidin-biotin-complex was used to detect the cytoplasm-expression and membrane-translocation of PKC θ in the splenocytes and thymocytes at 24 h after the whole body irradiation (WBI) with different doses of X-rays. Results The results showed that both low dose radiation and high dose radiation caused significant up-regulation of PKC θ in the cytoplasm and on the membrane of splenocytes and thymocytes (P<0.05 or P<0.01), both of which were more significant and dose-dependent in the thymocytes. The cytoplasm expression of PKC θ in the thymocytes peaks with 2 Gy irradiation and that in the splenocytes has not attained to the significant acme during the whole course, but with higher expression in both organs than those in control. The ratio of PKC θ membrane-positive cells in the thymus begins to rise dose-dependently from 0.1 Gy, peaks at 2 Gy and then descends until 10 Gy; that in the spleen is much higher than that in the thymus within the range of 0.075-0.5 Gy, but much lower within 0.5-10 Gy. Conclusions Ionizing radiation can induce the expression of PKC θ up-regulated significantly in the cytoplasm and on the membrane, which shows that PKC θ not only participates the maturation and activation of T cells, but also plays a role in other signal transductions at the same time and so has multiple biological activities. It is one of the key factors that make ionizing radiation affect the immunological function of organisms. |
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