| YU Hong-sheng,ZHU Jing-juan,SHANG Qing-jun.Effect of low dose pre-irradiation on DNA damage and genetic material damage caused by high dosage of cyclophosphamide[J].Chinese Journal of Radiological Medicine and Protection,2007,27(1):57-59 |
| Effect of low dose pre-irradiation on DNA damage and genetic material damage caused by high dosage of cyclophosphamide |
| Received:February 08, 2006 |
| DOI： |
| KeyWords:Low dose irradiation Cyclophosphamide DNA damage Micronucleus |
| FundProject:国家自然科学基金资助项目(30471457) |
| Author Name | Affiliation | E-mail | | YU Hong-sheng | Department of Oncology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China | qdhsyu@126.com | | ZHU Jing-juan | Department of Oncology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China | | | SHANG Qing-jun | Department of Oncology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China | | | 王卓敏 | Department of Oncology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China | | | 崔复宪 | Department of Oncology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China | |
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| Abstract:: |
| Objective To study the effect of low dose γ-rays pre-irradiation on the induction of DNA damage and genetic material damage in peripheral lymphocytes by high dosage of cyclophosphamide(CTX). Methods Male Kunming strain mice were randomly divided into five groups: control group, sham-irradiated group, low dose irradiated group(LDR group), cyclophosphamide chemotherapy group(CTX group) and low dose irradiation combined with chemotherapy group(LDR+CTX group). After being feeded for one week, all the mice were implanted subcutaneously with S180 cells in the left groin (control group excluded). On days 8 and 11, groups of LDR and LDR+CTX were administered with 75 mGy of whole-body irradiation, 30 h later groups CTX and LDR+CTX were injected intraperitoneally 3.0 mg cyclophosphamide. All the mice were sacrificed on day 13. DNA damage of the peripheral lymphocytes was analyzed using single cell gel electrophoresis(SCGE). Genetic material damage was analyzed using micronucleus frequency(MNF) of polychromatoerythrocytes(PCE) in bone marrow. Results 1 Compared with control group and sham-irradiated group, the DNA damage of peripheral lymphocytes in CTX group were increased significantly (P<0.01); 75 mGy pre-irradiation might decreased the DNA damage induced by chemotherapy significantly. 2 Compared with control group and sham-irradiated group, MNF of PCE in CTX group increased significantly(P<0.01),while 75 mGy pre-irradiation had a tendency to decline on MNF of PCE, but without statistical significance(P>0.05). Conclusions 1High-dosage of CTX chemotherapy can cause DNA damage in peripheral lymphocytes. 75 mGy γ-irradiation before chemotherapy may have certain protective effect on DNA damage. 2 CTX has potent mutagenic effect, giving remarkable rise to MNF of PCE. 75 mGy γ-ray pre-irradiation has not obvious protection against genetic toxicity of high-dose CTX chemotherapy. |
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