| WEI Wei,LI Wen-jian,SU Xu.Analysis of radiosensitivity and cell cycle of SMMC-7721 hepatoma cell line irradiated with different LET rays[J].Chinese Journal of Radiological Medicine and Protection,2007,27(1):53-56 |
| Analysis of radiosensitivity and cell cycle of SMMC-7721 hepatoma cell line irradiated with different LET rays |
| Received:February 28, 2006 |
| DOI： |
| KeyWords:Ionizing radiation SMMC-7721 cell line Radiosensitivity Cell cycle Cell apoptosis |
| FundProject:国家自然科学基金资助项目(10335050);科技部重大基础研究前期研究专项资助项目(2003CCB00200) |
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| Abstract:: |
| Objective To study the effect of different LET rays on change of radiosensitivity, cell cycle and apoptosis in SMMC-7721 hepatoma cells. Methods Exponentially grown SMMC-7721 cells were irradiated with 12C6+ ions and X-ray at doses of 0, 0.5, 1, 2, 4 and 8 Gy. The radiosensitivity of cells was assessed by means of the colony-forming assay. The DNA content, percentage of each cell-cycle phase and the apoptosis rate were determined with flow cytometry analysis. Results The survival fraction of SMMC-7721 hepatoma cells was remarkably lower after irradiation with 12C6+ ions than that of X-ray. With the increases of dose and repair time, the S phase arrest, G2/M phase arrest delay and the apoptosis in SMMC-7721 cells were significantly induced by 12C6+ ions. Conclusions 12C6+ ions can kill the SMMC-7721 cells and induce apoptosis more effectively in the cells than X-rays. |
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