| CHEN Ying,LIU Xiu-lin,YAO Bo.Biological dose estimation and Comet analysis of the victims in a high dose 60Co radiation accident[J].Chinese Journal of Radiological Medicine and Protection,2007,27(1):22-24 |
| Biological dose estimation and Comet analysis of the victims in a high dose 60Co radiation accident |
| Received:January 16, 2006 |
| DOI： |
| KeyWords:High dose irradiation Chromosome aberrations Biological dose Comet assay |
| FundProject: |
| Author Name | Affiliation | E-mail | | CHEN Ying | Beijing Institute of Radiation medicine, Beijing 100850, China | yingchen29@yahoo.com.cn | | LIU Xiu-lin | Beijing Institute of Radiation medicine, Beijing 100850, China | | | YAO Bo | Affiliated Hospital of Academy, Military Medical Science, Beijing 100071, China | | | 骆亿生 | Beijing Institute of Radiation medicine, Beijing 100850, China | | | 李友 | Beijing Institute of Radiation medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the methods of chromosome preparation in human peripheral blood and bone marrow after very high dose exposure and fit the dose-response curve of dicentrics and rings in the range of high doses over 6 Gy for estimating biological dose and detecting DNA damage in the victims of "10.21" accident. Methods The samples of peripheral blood and bone marrow in 2 victims were collected to prepare chromosome mataphases and dicentrics (multicentrics) + rings were counted. The dose-response curve and equation of human blood irradiated between 6-22 Gy in vitro were established and applied to assess biological dose of 2 victims. In addition, their DNA damages were tested by alkaline single cell gel electrophoresis. Results The dicentric+ring numbers of 4.47 per cell in victims B's peripheral blood lymphocytes and 9.15 per cell in victim A's bone marrow who had no mitosis in peripheral blood cell. The whole body average doses of victims B and A estimated by 6-22 Gy equation arrived at 9.4 Gy and 19.5 Gy, respectively. The serious DNA damages were expressed by small head and large tail comet figures. Conclusions The biological doses of 2 victims estimated by 6-22 Gy dose-response curve have reached the levels of extreme grave bone marrow and intestinal ARS, respectively. |
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