| LI Ping,YANG Zhi-hua,PAN Xiu-jie.Study on cytotoxicities induced by alpha particle irradiation combined with NNK treatment[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):583-586 |
| Study on cytotoxicities induced by alpha particle irradiation combined with NNK treatment |
| Received:January 10, 2006 |
| DOI： |
| KeyWords:Alpha particles irradiation NNK BEP2D Cell survival rate ROS Cell membrane damage |
| FundProject: |
| Author Name | Affiliation | E-mail | | LI Ping | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | zhumx@nic.bmi.ac.cn | | YANG Zhi-hua | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | PAN Xiu-jie | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 曹珍山 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 米娜 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 陈忠民 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 刘刚 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 魏菡 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 李慧颖 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 朱茂祥 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate cytotoxicities of alpha-particle irradiation combined with NNK treatment. Methods Exponentially growing immortalized human bronchial epithelial cells were divided into normal control group (NC), alpha particle irradiation group (α), NNK administration group (NNK), NNK administration (100 μg/ml) followed by alpha particle irradiation group (NNK+α), and alphaparticle irradiation followed by NNK administration (100 μg/ml) group (α+NNK). Cell survival fractions were measured by cloning rate of low-density plating cell. Ethidium bromide and 2′,7′-dichlorofluorescein, fluorescent products of the membrane-permeable dyes hydroethine and 2′,7′-dichloroflurescindiacetate were used to monitor the intracellular reactive oxygen species (ROS). Damage to membrane permeability was evaluated through testing LDH activity in medium. Results In the groups exposed to both alpha particles and NNK, the survival rates were significantly lower than that of the groups administrated with the same dose of alpha particles or NNK alone. The levels of intracellular ROS and the activity of LDH in medium were significantly higher than that of the groups administrated with the same dose of alpha particles or NNK alone. Subtracted the NNK effect, the survival rates of the groups received both alpha particle irradiation and NNK treatment were significantly lower than that of alpha particle irradiated only group. However, the intracellular ROS level and the activity of LDH in medium were significantly higher than that of alpha-particle irradiated only group. In addition, the survival rates of the cells in groups exposed to alpha particle irradiation followed by NNK administration were significantly lower than that of cells treated with NNK administration followed by alpha particle irradiation. Conclusions Alpha particle irradiation and NNK administration had synergisticity in cytotoxicity, and furthermore different schedules of the administration resulted in different survival rates. |
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